Development of a Rapid, Accurate and Sensitive Uv Spectroscopic Method for Estimation of Rivastigmine Tartrate Entrapped in Nanoparticles

Department of Pharmacy Sumandeep Vidyapeeth Vadodara( Gujarat)-391760 ABSTRACT A new simple, sensitive and selective UVspectrophotometric method was developed and dated for the estimation of Rivastigmine tartrate in bulk and in formulations. the method was applied for estimation of the drug present in the nanoparticles.RT was estimated at 264 nm in acetonitrile and in distill water.the linearity range was found to be 50-500ug/ml in acetonitrile according to the regression equation of absorbance=0.0015× (concentration in μg/ml) +0.0042and for distill water the regression equation of absorbance=0.0012× (concentration in μ/ml)+0.0027. The LOD for the assay of RT using ACN and distilled water was found to be 1.095 and 3.6148respectively. LOQ for the assay of RT using ACN and distilled water are found to be 1.3693 and 4.5643 respectively.The method was found to be accurate (mean percentage accuracy 99.65 )in ACN and method was found to be accurate (mean percentage accuracy 99.57) and precise with %RSD less than 1.97 (for intra-day) and less than 1.36 (for inter-day). The developed method was successfully employed for the determination of RT in pharmaceutical dosage forms (nanoparticles)